Journal: eLife
Article Title: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer
doi: 10.7554/eLife.44594
Figure Lengend Snippet:
Article Snippet: Using rat anti-strain Lai-IgG, rabbit anti-Rab5 or Rab11-IgG (Cell Signaling) or goat anti-transferrin receptor (TfR)-IgG (Santa Crus) as the primary antibody, AlexaFluor594-conjugated donkey anti-rat-IgG, AlexaFluor488-conjugated donkey anti-rabbit or goat-IgG or AlexaFluor405-conjugated donkey anti-rabbit-IgG (Abcam) as the second antibody, Lep-vesicle-Rab5 or Lep-vesicle-Rab11/TfR co-localization were detected by confocal microscopy (402/421 nm excitation/emission wavelengths for AlexaFluor405 detection) and the yellow or white FI reflecting the co-localization was measured as above.
Techniques: Control, Fluorescence, Western Blot, Bicinchoninic Acid Protein Assay, Negative Control